Sharp kink of DNA at psoralen-cross-link site deduced from crystal structure of psoralen-thymine monoadduct.
نویسندگان
چکیده
منابع مشابه
Recent advances in the synthesis and structure determination of site specifically psoralen-modified DNA oligonucleotides.
We have developed novel methods for the preparation of multimicromole quantities of extremely pure, uniquely photoadducted psoralen-DNA cross-links, furan-side monoadducted DNA and pyrone-side monoadducts. Psoralen cross-linked and furan-side monoadducted DNA were produced by employing high intensity argon ion and krypton ion lasers as light sources. Pyrone-side monoadducts were prepared by bas...
متن کاملThe crystal structures of psoralen cross-linked DNAs: drug-dependent formation of Holliday junctions.
The single-crystal structures are presented for two DNA sequences with the thymine bases covalently cross-linked across the complementary strands by 4'-hydroxymethyl-4,5',8-trimethylpsoralen (HMT). The HMT-adduct of d(CCGCTAGCGG) forms a psoralen-induced Holliday junction, showing for the first time the effect of this important class of chemotheraputics on the structure of the recombination int...
متن کاملRepair of 4,5',8-trimethylpsoralen monoadducts and cross-links by the Escherichia coli UvrABC endonuclease.
Using an oligonucleotide model substrate, we observed two unusual mechanisms of UvrABC endonuclease in the repair of 4,5',8-trimethylpsoralen monoadducts and crosslinks. (i) UvrABC endonuclease usually incises a psoralen monoadduct only on the damaged strand. However, for one of the monoadducts we studied, incision on the complementary undamaged strand was also observed at a very low frequency,...
متن کاملThe effects of covalent additions of a psoralen on transcription by E. coli RNA polymerase.
Synthetic DNA substrates containing a site-specifically engineered psoralen monoadduct or diadduct were used to characterize the response of the E. coli RNA polymerase elongation complex to these lesions. The psoralen derivative HMT (4'-hydroxymethyl-4,5', 8-trimethylpsoralen) was site specifically placed into two synthetic double-stranded DNA fragments each of which contained an E. coli RNA po...
متن کاملRecombinational and mutagenic repair of psoralen interstrand cross-links in Saccharomyces cerevisiae.
Psoralen photoreacts with DNA to form interstrand cross-links, which can be repaired by both nonmutagenic nucleotide excision repair and recombinational repair pathways and by mutagenic pathways. In the yeast Saccharomyces cerevisiae, psoralen cross-links are processed by nucleotide excision repair to form double-strand breaks (DSBs). In yeast, DSBs are repaired primarily by homologous recombin...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Cold Spring Harbor symposia on quantitative biology
دوره 47 Pt 1 شماره
صفحات -
تاریخ انتشار 1983